Initiation of transcription by T7 RNA polymerase as its natural promoters.
نویسندگان
چکیده
منابع مشابه
Structural Transitions Mediating Transcription Initiation by T7 RNA Polymerase
During transcription initiation, RNA polymerases appear to retain promoter interactions while transcribing short RNAs that are frequently released from the complex. Upon transition to elongation, the polymerase releases promoter and forms a stable elongation complex. Little is known about the changes in polymerase conformation or polymerase:DNA interactions that occur during this process. To ch...
متن کاملKinetic analysis of T7 RNA polymerase transcription initiation from promoters containing single-stranded regions.
T7 RNA polymerase is highly specific for the initiation of transcription from a relatively small consensus promoter sequence. Previous footprinting studies suggested that the enzyme binds specifically to a fully closed duplex form of the promoter, recognizing functional groups along one face of the helix [Muller, D. K., Martin, C. T., & Coleman, J. E. (1989) Biochemistry 28, 3306-3313]. Steady-...
متن کاملKinetic mechanism of GTP binding and RNA synthesis during transcription initiation by bacteriophage T7 RNA polymerase.
We have used stopped-flow and rapid chemical quench-flow methods to investigate the kinetics of the early steps during transcription initiation by bacteriophage T7 RNA polymerase. Most promoters of T7 RNA polymerase initiate with two GTPs. The kinetics of GTP binding was investigated by monitoring the fluorescence changes resulting from GTP binding to polymerase and fluorescent 2-aminopurine-co...
متن کاملM13 vectors with T7 polymerase promoters: transcription limited by oligonucleotides.
Synthetic sequences corresponding to T7 promoters have been cloned into the vectors H13mplO and M13mpl8 at the EcoRl site and into M13mpll and M13mpl9 at the Hlndlll site, creating mICE 10,11,18 and 19. These sequences of 33-base pairs include the region essential for activity of T7 promoters (1). The inserts preserve the g-galactosldase cloning marker and restore the initial unique restriction...
متن کاملTranscription of RNA templates by T7 RNA polymerase.
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was abl...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45929-7